The Bartholin's (BAHR toe linz) glands are located on each side of the vaginal opening. These glands secrete fluid that helps lubricate the vagina. Sometimes the openings of these glands become Bartholin Glands: If your vagina is a clock, it's 4:40 right now ladypartsblog.com Vulva Definition, Anatomy, & Function Britannica Arousal, lubrication AND the vestibular glands. The greater and lesser vestibular glands or bartholin and skene glands secrete fluid which provides lubrication. However, this is not the primary source of vaginal lubrication. Distinguishing Between Vaginal Discharge, Arousal Fluid, & Cervical Mucus